Intermediate

Part:BBa_J23028:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-07-25)


[trbCf][TT]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal NgoMIV site found at 587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

[trbCf][TT] Digest pSB1A2-J23014 (EcoRI/SpeI, Neb2, Small ) Paste into pSB1AKG0-B0015 (EcoRI/XbaI, Neb2, Large 2062) Product is pSB1A2-J23028.

References